Journal: Nature Communications
Article Title: Structural and mechanistic insights into a lysosomal membrane enzyme HGSNAT involved in Sanfilippo syndrome
doi: 10.1038/s41467-024-49614-1
Figure Lengend Snippet: a HGSNAT catalyzes the transmembrane acetylation of HS in the lysosome. The disaccharide repeating unit is represented by orange and magenta hexagons, while the terminal GlcN is shown as a green hexagon. Acetyl-CoA is represented by a yellow triangle (acetyl group) connected with a red circle (CoA). b Cryo-EM structure of human HGSNAT bound with acetyl-CoA. The two monomers within the dimer are colored in blue and orange respectively. Acetyl-CoA is colored in green. c Electrostatic potential of HGSNAT dimer. d Structure of HSGNAT dimer. e Topology map of the HGSNAT monomer colored in discrete colors corresponding to ( d ).
Article Snippet: The cDNA encoding the open reading frame of human HGSNAT (Uniprot: Q68CP4-2) was ordered from GenScript and cloned into the pEG-BacMam expression plasmid .
Techniques: Cryo-EM Sample Prep